Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2333776..2334620 Replicon chromosome
Accession NZ_CP024473
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name itaT Uniprot ID A0A4P7TPN5
Locus tag CPZ98_RS11460 Protein ID WP_005060796.1
Coordinates 2333776..2334174 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag CPZ98_RS11470 Protein ID WP_005053053.1
Coordinates 2334309..2334620 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS11445 (2328944) 2328944..2330674 - 1731 WP_000645011.1 hypothetical protein -
CPZ98_RS11450 (2330727) 2330727..2332880 - 2154 WP_005060811.1 SEL1-like repeat protein -
CPZ98_RS11455 (2333031) 2333031..2333728 + 698 WP_232046043.1 IS1 family transposase -
CPZ98_RS11460 (2333776) 2333776..2334174 - 399 WP_005060796.1 GNAT family N-acetyltransferase Toxin
CPZ98_RS11470 (2334309) 2334309..2334620 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
CPZ98_RS11475 (2334805) 2334805..2335695 - 891 WP_000971356.1 heme o synthase -
CPZ98_RS11480 (2335707) 2335707..2336036 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
CPZ98_RS11485 (2336036) 2336036..2336650 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
CPZ98_RS11490 (2336640) 2336640..2338631 - 1992 Protein_2214 cytochrome o ubiquinol oxidase subunit I -
CPZ98_RS11495 (2338653) 2338653..2339150 - 498 Protein_2215 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2333225..2333728 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(52-112)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14335.69 Da        Isoelectric Point: 9.1674

>T87714 WP_005060796.1 NZ_CP024473:c2334174-2333776 [Shigella flexneri 7b]
VRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKK
IHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 399 bp

>T87714 NZ_CP024473:c2334174-2333776 [Shigella flexneri 7b]
GTGCGTAATAGCGACTGTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGC
ATATTCGCTGGAAAGACAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCA
TGTTGGGGGGAGCGCGGAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAA
ATTCACCCGGCATTACCAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTT
TGTTCAGCTTTCAGCGACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT87714 WP_005053053.1 NZ_CP024473:c2334620-2334309 [Shigella flexneri 7b]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT87714 NZ_CP024473:c2334620-2334309 [Shigella flexneri 7b]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPN5

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References