Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2252766..2253460 Replicon chromosome
Accession NZ_CP024473
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CPZ98_RS11045 Protein ID WP_001263491.1
Coordinates 2252766..2253164 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag CPZ98_RS11050 Protein ID WP_000554759.1
Coordinates 2253167..2253460 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS11020 (2248105) 2248105..2248563 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CPZ98_RS11025 (2248825) 2248825..2250282 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
CPZ98_RS11030 (2250339) 2250339..2250899 - 561 Protein_2122 peptide chain release factor H -
CPZ98_RS11035 (2251015) 2251015..2252058 - 1044 WP_005060486.1 RNA ligase RtcB family protein -
CPZ98_RS11040 (2252304) 2252304..2252756 - 453 WP_001059846.1 GNAT family N-acetyltransferase -
CPZ98_RS11045 (2252766) 2252766..2253164 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CPZ98_RS11050 (2253167) 2253167..2253460 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CPZ98_RS11055 (2253512) 2253512..2254567 - 1056 WP_001226172.1 DNA polymerase IV -
CPZ98_RS11060 (2254638) 2254638..2255423 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
CPZ98_RS11065 (2255395) 2255395..2257107 + 1713 Protein_2129 flagellar biosynthesis protein FlhA -
CPZ98_RS11070 (2257161) 2257161..2257682 - 522 Protein_2130 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gmhA/lpcA 2212586..2278705 66119


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T87713 WP_001263491.1 NZ_CP024473:c2253164-2252766 [Shigella flexneri 7b]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T87713 NZ_CP024473:c2253164-2252766 [Shigella flexneri 7b]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT87713 WP_000554759.1 NZ_CP024473:c2253460-2253167 [Shigella flexneri 7b]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT87713 NZ_CP024473:c2253460-2253167 [Shigella flexneri 7b]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References