Detailed information of TA system TA087700

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87369..87633
• Replicon: plasmid pBU53M1
• Accession: NZ_CP024468
• Organism: Shigella dysenteriae strain BU53M1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CPZ97_RS25155
• Protein ID: WP_001387489.1
• Coordinates: 87369..87521 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	87573..87633 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ97_RS25125	83769..84383	+	615	WP_000578648.1	plasmid IncI1-type surface exclusion protein ExcA	-
CPZ97_RS25130	84481..84690	+	210	WP_001140543.1	hemolysin expression modulator Hha	-
CPZ97_RS25135	84934..85596	+	663	WP_042078443.1	plasmid IncI1-type surface exclusion protein ExcA	-
CPZ97_RS25140	85668..85877	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CPZ97_RS27885	86209..86385	+	177	WP_001054900.1	hypothetical protein	-
CPZ97_RS25145	86450..86746	-	297	WP_011264046.1	hypothetical protein	-
CPZ97_RS25150	87046..87297	+	252	WP_001291964.1	hypothetical protein	-
CPZ97_RS25155	87369..87521	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	87573..87633	+	61	NuclAT_0	-	Antitoxin
-	87573..87633	+	61	NuclAT_0	-	Antitoxin
-	87573..87633	+	61	NuclAT_0	-	Antitoxin
-	87573..87633	+	61	NuclAT_0	-	Antitoxin
CPZ97_RS25160	87836..88927	+	1092	WP_000426061.1	hypothetical protein	-
CPZ97_RS25165	89234..90442	+	1209	WP_000121263.1	IncI1-type conjugal transfer protein TrbA	-
CPZ97_RS25170	90461..91531	+	1071	WP_050197982.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / dfrA1 / ant(3'')-Ia / qacE / sul1 / tet(A)	-	1..115922	115922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T87700 WP_001387489.1 NZ_CP024468:c87521-87369 [Shigella dysenteriae]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87700 NZ_CP024468:c87521-87369 [Shigella dysenteriae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT87700 NZ_CP024468:87573-87633 [Shigella dysenteriae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin