Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2168189..2168816 Replicon chromosome
Accession NZ_CP024466
Organism Shigella dysenteriae strain BU53M1

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag CPZ97_RS12155 Protein ID WP_024258354.1
Coordinates 2168189..2168518 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag CPZ97_RS12160 Protein ID WP_000147081.1
Coordinates 2168508..2168816 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ97_RS12115 2163454..2164170 + 717 WP_001001693.1 arginine ABC transporter permease ArtQ -
CPZ97_RS12120 2164170..2164838 + 669 WP_000464490.1 arginine ABC transporter permease ArtM -
CPZ97_RS12125 2165129..2165449 + 321 Protein_2283 ABC transporter substrate-binding protein ArtJ -
CPZ97_RS12140 2166216..2166356 - 141 Protein_2285 HNH endonuclease -
CPZ97_RS12145 2166394..2167116 - 723 WP_134802076.1 DNA-binding protein -
CPZ97_RS12150 2167191..2167868 - 678 WP_000957540.1 phage antirepressor Ant -
CPZ97_RS12155 2168189..2168518 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ97_RS12160 2168508..2168816 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
CPZ97_RS12165 2168813..2169034 - 222 WP_001254231.1 NinE family protein -
CPZ97_RS12170 2169031..2169441 - 411 WP_000814621.1 recombination protein NinB -
CPZ97_RS12180 2170859..2171863 + 1005 Protein_2293 tyrosine-type recombinase/integrase -
CPZ97_RS12185 2171921..2172481 - 561 WP_001215754.1 DUF1175 domain-containing protein -
CPZ97_RS12190 2172478..2172819 - 342 Protein_2295 DUF2138 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2155226..2205665 50439
- inside IScluster/Tn - - 2158300..2170725 12425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T87694 WP_024258354.1 NZ_CP024466:2168189-2168518 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T87694 NZ_CP024466:2168189-2168518 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT87694 WP_000147081.1 NZ_CP024466:2168508-2168816 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT87694 NZ_CP024466:2168508-2168816 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References