Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2935..3527 Replicon plasmid pNP7-2
Accession NZ_CP024445
Organism Moraxella osloensis strain NP7

Toxin (Protein)


Gene name graT Uniprot ID A0A120KR99
Locus tag NP7_RS12005 Protein ID WP_062335123.1
Coordinates 3246..3527 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A120KRC1
Locus tag NP7_RS12000 Protein ID WP_062335121.1
Coordinates 2935..3231 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NP7_RS11985 56..1438 + 1383 WP_100271153.1 replication initiation protein -
NP7_RS11990 1517..2278 + 762 WP_100271154.1 ParA family protein -
NP7_RS11995 2275..2610 + 336 WP_100271155.1 hypothetical protein -
NP7_RS12000 2935..3231 - 297 WP_062335121.1 HigA family addiction module antidote protein Antitoxin
NP7_RS12005 3246..3527 - 282 WP_062335123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NP7_RS12010 3773..3928 + 156 Protein_5 recombinase family protein -
NP7_RS12015 3963..4463 + 501 WP_100271156.1 winged helix-turn-helix domain-containing protein -
NP7_RS12020 4451..5005 + 555 WP_145958820.1 IS630 family transposase -
NP7_RS12025 5020..5478 + 459 Protein_8 recombinase family protein -
NP7_RS12030 5581..5880 + 300 WP_099808668.1 type II toxin-antitoxin system RelE/ParE family toxin -
NP7_RS12035 5884..6225 + 342 WP_099808669.1 type II toxin-antitoxin system MqsA family antitoxin -
NP7_RS12040 7044..8168 + 1125 WP_100271158.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..134961 134961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(11-63)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10798.23 Da        Isoelectric Point: 6.8610

>T87659 WP_062335123.1 NZ_CP024445:c3527-3246 [Moraxella osloensis]
MIVNFACDKTKTLFTTGNTRHFSSIKKVAERKLLQLDNAITLEDLRSPPGNHLEALFGDREGQHSIRINDQWRICFVWTQ
EGPTEVEIVDYHR

Download         Length: 282 bp

>T87659 NZ_CP024445:c3527-3246 [Moraxella osloensis]
ATGATTGTTAATTTTGCATGTGATAAAACTAAAACACTTTTTACAACAGGCAACACACGTCATTTCTCTTCAATTAAAAA
AGTTGCTGAAAGAAAATTATTGCAATTAGATAATGCGATTACTTTAGAAGACTTGCGTTCTCCGCCAGGTAATCACCTTG
AAGCCCTATTTGGCGACAGAGAAGGTCAGCACAGTATTCGAATCAATGATCAATGGCGGATCTGCTTTGTATGGACACAA
GAAGGTCCGACAGAAGTAGAAATTGTAGATTACCATCGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11046.78 Da        Isoelectric Point: 7.2020

>AT87659 WP_062335121.1 NZ_CP024445:c3231-2935 [Moraxella osloensis]
MKNGMRPIHPGEILREDYLAPLEMSANALAKAIGVTPARINEIVREERGITADTALRLAKYFGGNAKFWLNLQTTYELRL
AETQDTHFLDKIMPLAFS

Download         Length: 297 bp

>AT87659 NZ_CP024445:c3231-2935 [Moraxella osloensis]
ATGAAAAATGGTATGAGACCTATTCATCCAGGTGAAATTTTGCGAGAAGACTATCTTGCACCGCTAGAAATGAGCGCCAA
TGCCTTAGCGAAGGCTATCGGCGTCACACCTGCCCGTATTAATGAAATCGTGCGCGAGGAACGTGGTATTACTGCTGATA
CGGCACTACGCCTTGCTAAATATTTTGGTGGTAATGCTAAGTTTTGGCTAAACTTACAAACCACTTACGAGCTACGACTA
GCTGAAACCCAAGATACGCATTTTTTAGATAAGATTATGCCATTAGCTTTCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A120KR99

Antitoxin

Source ID Structure
AlphaFold DB A0A120KRC1

References