Detailed information of TA system TA087652

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 109605..110031
• Replicon: plasmid p48896_1
• Accession: NZ_CP024430
• Organism: Klebsiella pneumoniae strain DA48896

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BKL84_RS29495
• Protein ID: WP_001312861.1
• Coordinates: 109605..109763 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	109807..110031 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKL84_RS27735	104628..104855	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
BKL84_RS27740	104992..105663	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
BKL84_RS27745	105857..106240	-	384	WP_001354030.1	relaxosome protein TraM	-
BKL84_RS27755	106563..107165	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
BKL84_RS27760	107462..108283	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BKL84_RS27765	108394..108690	-	297	WP_001272251.1	hypothetical protein	-
BKL84_RS30130	108990..109367	+	378	Protein_128	hypothetical protein	-
BKL84_RS29495	109605..109763	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	109807..110031	-	225	NuclAT_0	-	Antitoxin
-	109807..110031	-	225	NuclAT_0	-	Antitoxin
-	109807..110031	-	225	NuclAT_0	-	Antitoxin
-	109807..110031	-	225	NuclAT_0	-	Antitoxin
BKL84_RS27790	110043..110762	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BKL84_RS27795	110759..111193	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BKL84_RS27800	111262..113285	-	2024	Protein_132	ParB/RepB/Spo0J family partition protein	-
BKL84_RS27805	113346..113579	-	234	WP_000006003.1	DUF905 family protein	-
BKL84_RS27810	113637..114164	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-15 / aadA2 / dfrA12 / catA2	-	1..131243	131243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T87652 WP_001312861.1 NZ_CP024430:c109763-109605 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T87652 NZ_CP024430:c109763-109605 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT87652 NZ_CP024430:c110031-109807 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin