Detailed information of TA system TA087607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2313331..2313923
• Replicon: chromosome
• Accession: NZ_CP024311
• Organism: Rhizobium sp. NXC24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: NXC24_RS11395
• Protein ID: WP_104823382.1
• Coordinates: 2313642..2313923 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: NXC24_RS11390
• Protein ID: WP_104825122.1
• Coordinates: 2313331..2313630 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NXC24_RS11380	2308940..2309539	-	600	WP_104823380.1	transcriptional regulator	-
NXC24_RS11385	2309536..2313306	-	3771	WP_104823381.1	cobaltochelatase subunit CobN	-
NXC24_RS11390	2313331..2313630	-	300	WP_104825122.1	HigA family addiction module antidote protein	Antitoxin
NXC24_RS11395	2313642..2313923	-	282	WP_104823382.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NXC24_RS11400	2313981..2315030	-	1050	WP_104825123.1	cobalamin biosynthesis protein CobW	-
NXC24_RS11405	2315036..2315563	-	528	WP_104823383.1	bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase	-
NXC24_RS11410	2316286..2318082	+	1797	WP_104823384.1	chloride channel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (16-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10517.96 Da
• Isoelectric Point: 8.9312

>T87607 WP_104823382.1 NZ_CP024311:c2313923-2313642 [Rhizobium sp. NXC24]
VIVSYKGKFAEAVASGNVPKGFPADLVRSAQRKLTILNYAVVLDDLRSPPGNRLERLQGDRSGQYSIRINDQWRICFLWT
EAGPRDVEIVDYH

Nucleotide

• Length: 282 bp

>T87607 NZ_CP024311:c2313923-2313642 [Rhizobium sp. NXC24]
GTGATCGTTTCATACAAGGGGAAGTTCGCCGAAGCTGTGGCCTCAGGAAACGTGCCAAAGGGCTTTCCAGCCGACCTCGT
TCGTTCCGCGCAACGCAAGCTGACGATATTGAACTATGCGGTTGTGCTGGACGACCTGCGATCGCCTCCCGGCAATCGGC
TGGAGCGTTTGCAAGGTGATCGTTCTGGCCAGTATTCGATCAGAATCAACGATCAATGGCGCATTTGCTTCCTATGGACC
GAAGCTGGACCGCGTGATGTCGAGATCGTGGATTATCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10970.68 Da
• Isoelectric Point: 5.6939

>AT87607 WP_104825122.1 NZ_CP024311:c2313630-2313331 [Rhizobium sp. NXC24]
MTETKLPPIHPGEILREEYLKPLTMSAGALAKHLNVPRTRIERLVSEDTGVTPDTALRLARFFSTTPDFWMAMQTGYDLA
IAQAEKSDVFAAIKPLEAA

Nucleotide

• Length: 300 bp

>AT87607 NZ_CP024311:c2313630-2313331 [Rhizobium sp. NXC24]
ATGACCGAGACCAAACTTCCGCCGATCCATCCGGGTGAAATCTTGCGGGAGGAGTATCTGAAGCCATTGACGATGAGCGC
CGGTGCTTTGGCGAAACATCTTAACGTGCCTCGAACGCGTATTGAGAGGCTGGTTTCGGAGGACACCGGGGTCACGCCGG
ATACGGCACTTCGGCTCGCGCGGTTTTTCAGCACGACGCCAGATTTCTGGATGGCGATGCAAACGGGTTATGATCTCGCG
ATCGCGCAAGCGGAAAAGAGCGATGTATTCGCAGCCATCAAGCCGCTTGAAGCGGCTTAA