Detailed information of TA system TA087595

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105850..106267
• Replicon: plasmid unnamed2
• Accession: NZ_CP024291
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: A5960_RS27375
• Protein ID: WP_000775238.1
• Coordinates: 105850..106011 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	106076..106267 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS27330	101656..102039	-	384	WP_021521311.1	relaxosome protein TraM	-
A5960_RS27335	102370..102972	+	603	WP_077630563.1	transglycosylase SLT domain-containing protein	-
A5960_RS27340	103268..104089	-	822	WP_028985438.1	DUF945 domain-containing protein	-
A5960_RS27345	104207..104494	-	288	WP_000107526.1	hypothetical protein	-
A5960_RS27355	104650..104952	-	303	WP_001272236.1	hypothetical protein	-
A5960_RS28605	105252..105549	+	298	Protein_120	hypothetical protein	-
A5960_RS27375	105850..106011	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	106076..106267	+	192	-	-	Antitoxin
-	106076..106270	-	195	NuclAT_0	-	-
-	106076..106270	-	195	NuclAT_0	-	-
-	106076..106270	-	195	NuclAT_0	-	-
-	106076..106270	-	195	NuclAT_0	-	-
A5960_RS27380	106219..106533	-	315	Protein_122	theronine dehydrogenase	-
A5960_RS27385	106530..107249	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
A5960_RS27390	107246..107680	-	435	WP_000845927.1	conjugation system SOS inhibitor PsiB	-
A5960_RS27395	107735..109693	-	1959	WP_050864290.1	ParB/RepB/Spo0J family partition protein	-
A5960_RS27400	109758..109991	-	234	WP_000005988.1	DUF905 family protein	-
A5960_RS27410	110049..110570	-	522	WP_000290783.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..111697	111697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T87595 WP_000775238.1 NZ_CP024291:c106011-105850 [Escherichia coli O178:H19]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T87595 NZ_CP024291:c106011-105850 [Escherichia coli O178:H19]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 192 bp

>AT87595 NZ_CP024291:106076-106267 [Escherichia coli O178:H19]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGTAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACACTCACTTTCCCTGAAAAT
AATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin