Detailed information of TA system TA087593

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63736..63990
• Replicon: plasmid unnamed2
• Accession: NZ_CP024291
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: A5960_RS27095
• Protein ID: WP_032186826.1
• Coordinates: 63736..63942 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	63929..63990 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS27050	59269..59691	-	423	WP_028985400.1	Exc2 family lipoprotein	-
A5960_RS27055	59974..60489	+	516	Protein_66	transposase	-
A5960_RS27060	60820..61059	-	240	WP_000859018.1	MarR family transcriptional regulator	-
A5960_RS27080	62035..62892	-	858	WP_028985401.1	incFII family plasmid replication initiator RepA	-
A5960_RS27085	62885..62959	-	75	WP_062865987.1	RepA leader peptide Tap	-
A5960_RS27090	63195..63452	-	258	WP_000084404.1	replication regulatory protein RepA	-
A5960_RS27095	63736..63942	-	207	WP_032186826.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	63929..63990	+	62	NuclAT_1	-	Antitoxin
-	63929..63990	+	62	NuclAT_1	-	Antitoxin
-	63929..63990	+	62	NuclAT_1	-	Antitoxin
-	63929..63990	+	62	NuclAT_1	-	Antitoxin
A5960_RS28600	64165..64548	-	384	Protein_72	hypothetical protein	-
A5960_RS27110	64651..65110	-	460	Protein_73	thermonuclease family protein	-
A5960_RS27115	65853..66056	-	204	WP_032295436.1	hypothetical protein	-
A5960_RS27120	66211..66768	-	558	WP_028985402.1	fertility inhibition protein FinO	-
A5960_RS27125	66823..67569	-	747	WP_028985403.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..111697	111697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7822.32 Da
• Isoelectric Point: 8.8807

>T87593 WP_032186826.1 NZ_CP024291:c63942-63736 [Escherichia coli O178:H19]
MKYLNTTDCSLFLAERSKFMTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87593 NZ_CP024291:c63942-63736 [Escherichia coli O178:H19]
ATGAAGTATCTCAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTAATTGG
GTTGCTCGCTGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT87593 NZ_CP024291:63929-63990 [Escherichia coli O178:H19]
TTGAGATACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin