Detailed information of TA system TA087586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3742612..3743214
• Replicon: chromosome
• Accession: NZ_CP024289
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A5960_RS19555
• Protein ID: WP_000897305.1
• Coordinates: 3742903..3743214 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A5960_RS19550
• Protein ID: WP_000356397.1
• Coordinates: 3742612..3742902 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS19520	3738538..3739440	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
A5960_RS19525	3739437..3740072	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A5960_RS19530	3740069..3740998	+	930	WP_000027707.1	formate dehydrogenase accessory protein FdhE	-
A5960_RS19535	3741328..3741570	-	243	WP_001086388.1	hypothetical protein	-
A5960_RS19540	3741789..3742007	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
A5960_RS19550	3742612..3742902	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A5960_RS19555	3742903..3743214	-	312	WP_000897305.1	hypothetical protein	Toxin
A5960_RS19560	3743443..3744351	+	909	WP_001162704.1	alpha/beta hydrolase	-
A5960_RS19565	3744415..3745356	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
A5960_RS19570	3745401..3745838	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A5960_RS19575	3745835..3746707	-	873	WP_000920762.1	virulence factor BrkB family protein	-
A5960_RS19580	3746701..3747300	-	600	WP_001295269.1	glucose-1-phosphatase	-
A5960_RS19585	3747399..3748184	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87586 WP_000897305.1 NZ_CP024289:c3743214-3742903 [Escherichia coli O178:H19]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87586 NZ_CP024289:c3743214-3742903 [Escherichia coli O178:H19]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87586 WP_000356397.1 NZ_CP024289:c3742902-3742612 [Escherichia coli O178:H19]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87586 NZ_CP024289:c3742902-3742612 [Escherichia coli O178:H19]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7