Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2830307..2830848 Replicon chromosome
Accession NZ_CP024289
Organism Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag A5960_RS14980 Protein ID WP_000615976.1
Coordinates 2830570..2830848 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A5960_RS14975 Protein ID WP_000729704.1
Coordinates 2830307..2830567 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5960_RS14950 2825343..2826482 + 1140 WP_000785664.1 flagellar type III secretion system protein FlhB -
A5960_RS14955 2826466..2828559 + 2094 WP_001140827.1 flagellar biosynthesis protein FlhA -
A5960_RS14960 2828664..2828942 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A5960_RS14965 2828935..2829291 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A5960_RS14970 2829348..2830121 - 774 WP_000543899.1 C40 family peptidase -
A5960_RS14975 2830307..2830567 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A5960_RS14980 2830570..2830848 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A5960_RS14985 2831004..2831744 + 741 WP_001225679.1 murein L,D-transpeptidase -
A5960_RS14990 2831715..2832482 - 768 WP_000333380.1 class II glutamine amidotransferase -
A5960_RS14995 2832688..2833266 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T87582 WP_000615976.1 NZ_CP024289:2830570-2830848 [Escherichia coli O178:H19]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87582 NZ_CP024289:2830570-2830848 [Escherichia coli O178:H19]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87582 WP_000729704.1 NZ_CP024289:2830307-2830567 [Escherichia coli O178:H19]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87582 NZ_CP024289:2830307-2830567 [Escherichia coli O178:H19]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References