Detailed information of TA system TA087562

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81599..82022
• Replicon: plasmid unnamed4
• Accession: NZ_CP024286
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: CCF14_RS26895
• Protein ID: WP_000775238.1
• Coordinates: 81599..81760 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81806..82022 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS26860	76973..77200	-	228	WP_019842732.1	conjugal transfer relaxosome protein TraY	-
CCF14_RS26865	77319..77966	-	648	WP_099588251.1	transcriptional regulator TraJ family protein	-
CCF14_RS26870	78161..78544	-	384	WP_019842734.1	relaxosome protein TraM	-
CCF14_RS26875	78820..79467	+	648	WP_157760422.1	transglycosylase SLT domain-containing protein	-
CCF14_RS26880	79762..80583	-	822	WP_157760423.1	DUF945 domain-containing protein	-
CCF14_RS28325	80762..81361	+	600	Protein_77	hypothetical protein	-
CCF14_RS26895	81599..81760	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	81806..82022	-	217	NuclAT_0	-	Antitoxin
-	81806..82022	-	217	NuclAT_0	-	Antitoxin
-	81806..82022	-	217	NuclAT_0	-	Antitoxin
-	81806..82022	-	217	NuclAT_0	-	Antitoxin
CCF14_RS26900	82034..82753	-	720	Protein_79	plasmid SOS inhibition protein A	-
CCF14_RS26905	82750..83184	-	435	WP_099588253.1	conjugation system SOS inhibitor PsiB	-
CCF14_RS26910	83239..84767	-	1529	Protein_81	ParB/RepB/Spo0J family partition protein	-
CCF14_RS26915	84831..85064	-	234	WP_099588254.1	DUF905 family protein	-
CCF14_RS26920	85122..85655	-	534	WP_099588255.1	single-stranded DNA-binding protein	-
CCF14_RS26930	86049..86411	+	363	Protein_84	pilus protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeI / faeH / faeF / faeE / faeD / faeC / vat / papD / papC	1..113727	113727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T87562 WP_000775238.1 NZ_CP024286:c81760-81599 [Escherichia albertii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T87562 NZ_CP024286:c81760-81599 [Escherichia albertii]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGTTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 217 bp

>AT87562 NZ_CP024286:c82022-81806 [Escherichia albertii]
TCACACAGATTACCTGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin