Detailed information of TA system TA087560

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42384..42638
• Replicon: plasmid unnamed4
• Accession: NZ_CP024286
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CCF14_RS26665
• Protein ID: WP_001351576.1
• Coordinates: 42384..42590 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	42577..42638 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS26625	37976..38833	-	858	WP_157760420.1	incFII family plasmid replication initiator RepA	-
CCF14_RS26630	38826..38900	-	75	WP_032144212.1	RepA leader peptide Tap	-
CCF14_RS26640	39146..39394	-	249	WP_099588233.1	replication regulatory protein RepA	-
CCF14_RS26645	39465..41035	-	1571	Protein_32	IS66 family transposase	-
CCF14_RS26650	41055..41402	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CCF14_RS26655	41402..42079	-	678	WP_001682408.1	IS66-like element accessory protein TnpA	-
CCF14_RS26665	42384..42590	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	42577..42638	+	62	NuclAT_1	-	Antitoxin
-	42577..42638	+	62	NuclAT_1	-	Antitoxin
-	42577..42638	+	62	NuclAT_1	-	Antitoxin
-	42577..42638	+	62	NuclAT_1	-	Antitoxin
CCF14_RS26675	42815..43048	-	234	Protein_36	DUF2726 domain-containing protein	-
CCF14_RS26680	43036..43272	-	237	Protein_37	thermonuclease family protein	-
CCF14_RS26690	43511..43723	-	213	WP_099588235.1	hypothetical protein	-
CCF14_RS26695	43861..44421	-	561	WP_099588236.1	fertility inhibition protein FinO	-
CCF14_RS26700	44476..45222	-	747	WP_099588237.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeI / faeH / faeF / faeE / faeD / faeC / vat / papD / papC	1..113727	113727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T87560 WP_001351576.1 NZ_CP024286:c42590-42384 [Escherichia albertii]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87560 NZ_CP024286:c42590-42384 [Escherichia albertii]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GCTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT87560 NZ_CP024286:42577-42638 [Escherichia albertii]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin