Detailed information of TA system TA087553

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11536..11800
• Replicon: plasmid unnamed3
• Accession: NZ_CP024285
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: CCF14_RS25640
• Protein ID: WP_001331364.1
• Coordinates: 11536..11688 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	11743..11800 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS25620	6814..8976	+	2163	WP_000698351.1	DotA/TraY family protein	-
CCF14_RS25625	9041..9703	+	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
CCF14_RS25630	9775..9984	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CCF14_RS28520	10376..10552	+	177	WP_001054904.1	hypothetical protein	-
CCF14_RS25635	10617..10913	-	297	WP_001275298.1	hypothetical protein	-
CCF14_RS25640	11536..11688	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	11743..11800	+	58	NuclAT_0	-	Antitoxin
-	11743..11800	+	58	NuclAT_0	-	Antitoxin
-	11743..11800	+	58	NuclAT_0	-	Antitoxin
-	11743..11800	+	58	NuclAT_0	-	Antitoxin
CCF14_RS25645	11980..13188	+	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
CCF14_RS25650	13207..14277	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
CCF14_RS25655	14270..16561	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / tet(A)	htpB	1..127606	127606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T87553 WP_001331364.1 NZ_CP024285:c11688-11536 [Escherichia albertii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87553 NZ_CP024285:c11688-11536 [Escherichia albertii]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT87553 NZ_CP024285:11743-11800 [Escherichia albertii]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin