Detailed information of TA system TA087550

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36230..36499
• Replicon: plasmid unnamed2
• Accession: NZ_CP024284
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CCF14_RS25400
• Protein ID: WP_001312861.1
• Coordinates: 36341..36499 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36230..36295 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS28305	31519..31731	+	213	WP_001208963.1	hypothetical protein	-
CCF14_RS28510	31767..31973	+	207	WP_000547971.1	hypothetical protein	-
CCF14_RS25375	31999..32538	+	540	WP_009426937.1	single-stranded DNA-binding protein	-
CCF14_RS25380	32600..32833	+	234	WP_000006030.1	DUF905 family protein	-
CCF14_RS25385	32898..34856	+	1959	WP_099588204.1	ParB/RepB/Spo0J family partition protein	-
CCF14_RS25390	34911..35345	+	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
CCF14_RS25395	35342..36061	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CCF14_RS28310	36073..36261	-	189	WP_001299721.1	hypothetical protein	-
-	36073..36297	+	225	NuclAT_0	-	-
-	36073..36297	+	225	NuclAT_0	-	-
-	36073..36297	+	225	NuclAT_0	-	-
-	36073..36297	+	225	NuclAT_0	-	-
-	36230..36295	+	66	-	-	Antitoxin
CCF14_RS25400	36341..36499	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CCF14_RS25410	36942..37154	+	213	WP_100030856.1	hypothetical protein	-
CCF14_RS28515	37190..37396	+	207	WP_000547971.1	hypothetical protein	-
CCF14_RS25420	37421..37708	+	288	WP_000107535.1	hypothetical protein	-
CCF14_RS25425	37826..38647	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CCF14_RS25430	38944..39534	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-
CCF14_RS25440	39869..40252	+	384	WP_021559009.1	relaxosome protein TraM	-
CCF14_RS25445	40446..41117	+	672	WP_032083167.1	conjugal transfer transcriptional regulator TraJ	-
CCF14_RS25450	41254..41481	+	228	WP_000089265.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..59626	59626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T87550 WP_001312861.1 NZ_CP024284:36341-36499 [Escherichia albertii]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T87550 NZ_CP024284:36341-36499 [Escherichia albertii]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT87550 NZ_CP024284:36230-36295 [Escherichia albertii]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin