Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1727547..1728259 Replicon chromosome
Accession NZ_CP024282
Organism Escherichia albertii strain 2014C-4356

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CCF14_RS08710 Protein ID WP_059259167.1
Coordinates 1727957..1728259 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CCF14_RS08705 Protein ID WP_000806447.1
Coordinates 1727547..1727885 (-) Length 113 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCF14_RS08675 1722587..1724569 + 1983 WP_059215192.1 TonB-dependent heme/hemoglobin receptor ChuA/ShuA -
CCF14_RS08680 1724618..1725646 + 1029 WP_001110408.1 hematinate-forming heme oxygenase ChuS -
CCF14_RS08685 1725718..1726248 - 531 WP_059257619.1 LuxR family transcriptional regulator -
CCF14_RS08695 1726395..1726961 - 567 WP_099587876.1 outer membrane lipoprotein Slp -
CCF14_RS08700 1727310..1727490 - 181 Protein_1591 hypothetical protein -
CCF14_RS08705 1727547..1727885 - 339 WP_000806447.1 HigA family addiction module antidote protein Antitoxin
CCF14_RS08710 1727957..1728259 - 303 WP_059259167.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CCF14_RS08715 1728423..1728902 + 480 WP_099587877.1 hypothetical protein -
CCF14_RS08720 1728992..1729165 - 174 WP_044708027.1 hypothetical protein -
CCF14_RS08725 1729193..1729276 + 84 WP_077819772.1 Damage inducible protein -
CCF14_RS08730 1729330..1730682 - 1353 WP_054410173.1 glutathione-disulfide reductase -
CCF14_RS08735 1730754..1731596 - 843 WP_054410171.1 23S rRNA (adenine(2030)-N(6))-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(8-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11889.62 Da        Isoelectric Point: 9.8664

>T87528 WP_059259167.1 NZ_CP024282:c1728259-1727957 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKMPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Download         Length: 303 bp

>T87528 NZ_CP024282:c1728259-1727957 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATGCCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAAGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin


Download         Length: 113 a.a.        Molecular weight: 13010.90 Da        Isoelectric Point: 5.2075

>AT87528 WP_000806447.1 NZ_CP024282:c1727885-1727547 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNSRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Download         Length: 339 bp

>AT87528 NZ_CP024282:c1727885-1727547 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAGTCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References