Detailed information of TA system TA087525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1281055..1281657
• Replicon: chromosome
• Accession: NZ_CP024282
• Organism: Escherichia albertii strain 2014C-4356

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8H9KB27
• Locus tag: CCF14_RS06540
• Protein ID: WP_000897299.1
• Coordinates: 1281346..1281657 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCF14_RS06535
• Protein ID: WP_000356397.1
• Coordinates: 1281055..1281345 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF14_RS06490	1276475..1277377	+	903	WP_059259645.1	formate dehydrogenase O subunit beta	-
CCF14_RS06495	1277374..1278009	+	636	WP_000829019.1	formate dehydrogenase cytochrome b556 subunit	-
CCF14_RS06500	1278006..1278935	+	930	WP_000027704.1	formate dehydrogenase accessory protein FdhE	-
CCF14_RS06505	1278972..1279331	-	360	Protein_1194	type II toxin-antitoxin system VapC family toxin	-
CCF14_RS06510	1279331..1279573	-	243	Protein_1195	CopG family transcriptional regulator	-
CCF14_RS06515	1279801..1280019	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
CCF14_RS06525	1280418..1280696	-	279	WP_032278934.1	hypothetical protein	-
CCF14_RS06530	1280758..1280970	-	213	WP_000197774.1	hypothetical protein	-
CCF14_RS06535	1281055..1281345	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CCF14_RS06540	1281346..1281657	-	312	WP_000897299.1	hypothetical protein	Toxin
CCF14_RS06545	1281886..1282794	+	909	WP_010342110.1	alpha/beta hydrolase	-
CCF14_RS06550	1282860..1283801	-	942	WP_059259649.1	fatty acid biosynthesis protein FabY	-
CCF14_RS06555	1283846..1284283	-	438	WP_000560979.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CCF14_RS06560	1284280..1285152	-	873	WP_000916663.1	virulence factor BrkB family protein	-
CCF14_RS06565	1285146..1285745	-	600	WP_002460585.1	glucose-1-phosphatase	-
CCF14_RS06570	1285844..1286629	-	786	WP_000059671.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12251.24 Da
• Isoelectric Point: 9.7791

>T87525 WP_000897299.1 NZ_CP024282:c1281657-1281346 [Escherichia albertii]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWFSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87525 NZ_CP024282:c1281657-1281346 [Escherichia albertii]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGTTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87525 WP_000356397.1 NZ_CP024282:c1281345-1281055 [Escherichia albertii]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87525 NZ_CP024282:c1281345-1281055 [Escherichia albertii]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG