Detailed information of TA system TA087513

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67178..67448
• Replicon: plasmid unnamed3
• Accession: NZ_CP024281
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CS031_RS27460
• Protein ID: WP_001312861.1
• Coordinates: 67290..67448 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67178..67241 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS28165	62248..62502	+	255	WP_072254791.1	hypothetical protein	-
CS031_RS27435	62972..63499	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CS031_RS27440	63555..63788	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CS031_RS27445	63847..65805	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CS031_RS27450	65860..66294	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CS031_RS27455	66291..67010	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CS031_RS27960	67022..67210	-	189	WP_001299721.1	hypothetical protein	-
-	67022..67246	+	225	NuclAT_0	-	-
-	67022..67246	+	225	NuclAT_0	-	-
-	67022..67246	+	225	NuclAT_0	-	-
-	67022..67246	+	225	NuclAT_0	-	-
-	67178..67241	-	64	-	-	Antitoxin
CS031_RS27460	67290..67448	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CS031_RS27485	68367..68654	+	288	WP_000107537.1	hypothetical protein	-
CS031_RS27490	68774..69595	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CS031_RS27495	69892..70539	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
CS031_RS27500	70825..71208	+	384	WP_001151564.1	relaxosome protein TraM	-
CS031_RS27505	71402..72088	+	687	WP_000332487.1	PAS domain-containing protein	-
CS031_RS27510	72182..72409	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / qacE / sul1	-	1..84894	84894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T87513 WP_001312861.1 NZ_CP024281:67290-67448 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T87513 NZ_CP024281:67290-67448 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT87513 NZ_CP024281:c67241-67178 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin