Detailed information of TA system TA087509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4364990..4365592
• Replicon: chromosome
• Accession: NZ_CP024278
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CS031_RS22775
• Protein ID: WP_000897305.1
• Coordinates: 4365281..4365592 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CS031_RS22770
• Protein ID: WP_000356397.1
• Coordinates: 4364990..4365280 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS22740	4360916..4361818	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CS031_RS22745	4361815..4362450	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CS031_RS22750	4362447..4363376	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CS031_RS22755	4363706..4363948	-	243	WP_001086388.1	hypothetical protein	-
CS031_RS22760	4364167..4364385	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CS031_RS22770	4364990..4365280	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CS031_RS22775	4365281..4365592	-	312	WP_000897305.1	hypothetical protein	Toxin
CS031_RS22780	4365821..4366729	+	909	WP_001162704.1	alpha/beta hydrolase	-
CS031_RS22785	4366793..4367734	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CS031_RS22790	4367779..4368216	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CS031_RS22795	4368213..4369085	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CS031_RS22800	4369079..4369678	-	600	WP_001315111.1	glucose-1-phosphatase	-
CS031_RS22805	4369777..4370562	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87509 WP_000897305.1 NZ_CP024278:c4365592-4365281 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87509 NZ_CP024278:c4365592-4365281 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87509 WP_000356397.1 NZ_CP024278:c4365280-4364990 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87509 NZ_CP024278:c4365280-4364990 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7