Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1451252..1451754 Replicon chromosome
Accession NZ_CP024278
Organism Escherichia coli strain ATCC 43896

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag CS031_RS07515 Protein ID WP_000767835.1
Coordinates 1451500..1451754 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CS031_RS07510 Protein ID WP_001259255.1
Coordinates 1451252..1451503 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS031_RS07480 1446429..1447496 - 1068 WP_000080072.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CS031_RS07485 1447496..1448566 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
CS031_RS07490 1448563..1449867 - 1305 WP_096974555.1 histidinol dehydrogenase -
CS031_RS07495 1449873..1450772 - 900 WP_000131774.1 ATP phosphoribosyltransferase -
CS031_RS07505 1450918..1450968 - 51 WP_001364200.1 his operon leader peptide -
CS031_RS07510 1451252..1451503 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CS031_RS07515 1451500..1451754 + 255 WP_000767835.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CS031_RS07520 1451837..1452661 + 825 WP_000754737.1 SDR family oxidoreductase -
CS031_RS07525 1452707..1453636 + 930 WP_000803351.1 LysR family transcriptional regulator -
CS031_RS07530 1453851..1453913 + 63 WP_010723108.1 membrane protein YoeI -
CS031_RS07535 1453903..1455261 + 1359 WP_032327197.1 putrescine/proton symporter PlaP -
CS031_RS07540 1455440..1456498 + 1059 WP_000492320.1 transport protein YeeE -
CS031_RS07545 1456512..1456739 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.51 Da        Isoelectric Point: 7.2751

>T87492 WP_000767835.1 NZ_CP024278:1451500-1451754 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87492 NZ_CP024278:1451500-1451754 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87492 WP_001259255.1 NZ_CP024278:1451252-1451503 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87492 NZ_CP024278:1451252-1451503 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References