Detailed information of TA system TA087463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 400304..400998
• Replicon: chromosome
• Accession: NZ_CP024275
• Organism: Escherichia coli O6:H16 strain M9682-C1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: COG40_RS01995
• Protein ID: WP_001263493.1
• Coordinates: 400600..400998 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: COG40_RS01990
• Protein ID: WP_000554757.1
• Coordinates: 400304..400597 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG40_RS01965	396118..396585	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
COG40_RS01970	396605..397324	+	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
COG40_RS01975	397337..398200	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
COG40_RS01980	398203..399126	+	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
COG40_RS01985	399197..400252	+	1056	WP_001226164.1	DNA polymerase IV	-
COG40_RS01990	400304..400597	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
COG40_RS01995	400600..400998	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
COG40_RS02000	401008..401460	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
COG40_RS02005	401706..401909	+	204	Protein_371	RNA ligase RtcB family protein	-
COG40_RS02010	401908..402429	+	522	Protein_372	peptide chain release factor H	-
COG40_RS02015	402486..403943	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
COG40_RS02020	404204..404662	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
COG40_RS02025	404754..405998	+	1245	WP_000189541.1	esterase FrsA	-
-	405258..405338	+	81	NuclAT_12	-	-
-	405258..405338	+	81	NuclAT_12	-	-
-	405258..405338	+	81	NuclAT_12	-	-
-	405258..405338	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87463 WP_001263493.1 NZ_CP024275:400600-400998 [Escherichia coli O6:H16]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87463 NZ_CP024275:400600-400998 [Escherichia coli O6:H16]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87463 WP_000554757.1 NZ_CP024275:400304-400597 [Escherichia coli O6:H16]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87463 NZ_CP024275:400304-400597 [Escherichia coli O6:H16]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6