Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3755996..3756537 Replicon chromosome
Accession NZ_CP024269
Organism Escherichia coli strain F8111-1SC3

Toxin (Protein)


Gene name yafQ Uniprot ID A0A3W4DAB6
Locus tag CCV27_RS18905 Protein ID WP_024171431.1
Coordinates 3755996..3756274 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CCV27_RS18910 Protein ID WP_000729704.1
Coordinates 3756277..3756537 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCV27_RS18890 3753579..3754157 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CCV27_RS18895 3754363..3755129 + 767 Protein_3473 class II glutamine amidotransferase -
CCV27_RS18900 3755100..3755840 - 741 WP_001225679.1 murein L,D-transpeptidase -
CCV27_RS18905 3755996..3756274 - 279 WP_024171431.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CCV27_RS18910 3756277..3756537 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CCV27_RS18915 3756747..3757496 + 750 Protein_3477 C40 family peptidase -
CCV27_RS18920 3757672..3758169 + 498 WP_001612413.1 REP-associated tyrosine transposase RayT -
CCV27_RS18925 3758356..3760068 - 1713 Protein_3479 flagellar biosynthesis protein FlhA -
CCV27_RS18930 3760040..3760825 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - tssA / gmhA/lpcA 3737800..3758169 20369


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10829.68 Da        Isoelectric Point: 10.3349

>T87434 WP_024171431.1 NZ_CP024269:c3756274-3755996 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVKPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87434 NZ_CP024269:c3756274-3755996 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCAAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87434 WP_000729704.1 NZ_CP024269:c3756537-3756277 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87434 NZ_CP024269:c3756537-3756277 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W4DAB6

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References