Detailed information of TA system TA087415

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 120443..120814
• Replicon: chromosome
• Accession: NZ_CP024269
• Organism: Escherichia coli strain F8111-1SC3

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A7W3G0W6
• Locus tag: CCV27_RS00665
• Protein ID: WP_072130784.1
• Coordinates: 120620..120814 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	120443..120621 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCV27_RS00640	116398..117771	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
CCV27_RS00645	117900..118835	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CCV27_RS00650	118887..120122	-	1236	WP_024171493.1	site-specific integrase	-
CCV27_RS00655	120124..120339	-	216	WP_000079604.1	excisionase XisR	-
CCV27_RS00660	120439..120627	-	189	WP_001302840.1	DUF1187 family protein	-
-	120443..120621	+	179	NuclAT_0	-	Antitoxin
-	120443..120621	+	179	NuclAT_0	-	Antitoxin
-	120443..120621	+	179	NuclAT_0	-	Antitoxin
-	120443..120621	+	179	NuclAT_0	-	Antitoxin
CCV27_RS00665	120620..120814	-	195	WP_072130784.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CCV27_RS00670	120871..121704	-	834	WP_000166322.1	recombination protein RecT	-
CCV27_RS00675	121697..124372	-	2676	WP_001718192.1	exodeoxyribonuclease VIII	-
CCV27_RS00680	124471..124746	-	276	WP_001594001.1	phage protein	-
CCV27_RS00685	124821..124997	-	177	WP_001594002.1	phage protein	-
CCV27_RS00690	124991..125212	-	222	WP_000560220.1	killing protein KilR	-
CCV27_RS00695	125633..125785	-	153	WP_001594003.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	118887..142613	23726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7135.00 Da
• Isoelectric Point: 8.0115

>T87415 WP_072130784.1 NZ_CP024269:c120814-120620 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKDISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTENINGGVHV

Nucleotide

• Length: 195 bp

>T87415 NZ_CP024269:c120814-120620 [Escherichia coli]
TTGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGACATTTCAGGATATTACCG
GGCAAAATGCAACGGGTGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCGCTCGAAAGATGGAATAAACGAA
CCACTGAAAATATTAATGGAGGCGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT87415 NZ_CP024269:120443-120621 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATTGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7W3G0W6

Antitoxin