Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1757292..1758019 Replicon chromosome
Accession NZ_CP024266
Organism Escherichia coli O6:H16 strain F6699

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag COG39_RS08600 Protein ID WP_000550189.1
Coordinates 1757292..1757606 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag COG39_RS08605 Protein ID WP_000560266.1
Coordinates 1757603..1758019 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG39_RS08580 (1753450) 1753450..1754436 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
COG39_RS08585 (1754515) 1754515..1755207 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
COG39_RS08590 (1755284) 1755284..1755787 - 504 WP_001333820.1 M48 family metallopeptidase -
COG39_RS08595 (1755872) 1755872..1757008 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
COG39_RS08600 (1757292) 1757292..1757606 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
COG39_RS08605 (1757603) 1757603..1758019 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
COG39_RS08610 (1758064) 1758064..1760082 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
COG39_RS08615 (1760308) 1760308..1762659 - 2352 WP_000695470.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T87397 WP_000550189.1 NZ_CP024266:1757292-1757606 [Escherichia coli O6:H16]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T87397 NZ_CP024266:1757292-1757606 [Escherichia coli O6:H16]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT87397 WP_000560266.1 NZ_CP024266:1757603-1758019 [Escherichia coli O6:H16]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT87397 NZ_CP024266:1757603-1758019 [Escherichia coli O6:H16]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References