Detailed information of TA system TA087391

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84026..84280
• Replicon: plasmid unnamed2
• Accession: NZ_CP024265
• Organism: Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: COG38_RS26200
• Protein ID: WP_001362723.1
• Coordinates: 84026..84232 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	84224..84280 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG38_RS26160	80421..81067	-	647	Protein_90	IS630 family transposase	-
COG38_RS26165	81068..81469	+	402	Protein_91	IS91 family transposase	-
COG38_RS26180	82332..83189	-	858	WP_001401515.1	incFII family plasmid replication initiator RepA	-
COG38_RS26185	83182..83256	-	75	WP_001365705.1	RepA leader peptide Tap	-
COG38_RS26195	83482..83742	-	261	WP_000083817.1	replication regulatory protein RepA	-
COG38_RS26200	84026..84232	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84224..84280	+	57	NuclAT_1	-	Antitoxin
-	84224..84280	+	57	NuclAT_1	-	Antitoxin
-	84224..84280	+	57	NuclAT_1	-	Antitoxin
-	84224..84280	+	57	NuclAT_1	-	Antitoxin
COG38_RS26210	84524..84706	+	183	WP_001393357.1	hypothetical protein	-
COG38_RS26215	84930..85661	-	732	WP_001717613.1	type-F conjugative transfer system pilin acetylase TraX	-
COG38_RS26220	85658..86204	-	547	Protein_98	DUF2726 domain-containing protein	-
COG38_RS26225	86222..89059	-	2838	Protein_99	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeE / faeD / cofA / cssA / estIa	1..150389	150389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T87391 WP_001362723.1 NZ_CP024265:c84232-84026 [Escherichia coli O169:H41]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87391 NZ_CP024265:c84232-84026 [Escherichia coli O169:H41]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACCTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT87391 NZ_CP024265:84224-84280 [Escherichia coli O169:H41]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCCTTTCT

Structures

Antitoxin