Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4504136..4504677 Replicon chromosome
Accession NZ_CP024263
Organism Escherichia coli O169:H41 strain F6326-C1

Toxin (Protein)


Gene name yafQ Uniprot ID A0A3W4DAB6
Locus tag COG38_RS22995 Protein ID WP_024171431.1
Coordinates 4504136..4504414 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag COG38_RS23000 Protein ID WP_000729704.1
Coordinates 4504417..4504677 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG38_RS22980 4501718..4502296 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
COG38_RS22985 4502502..4503269 + 768 WP_000333380.1 class II glutamine amidotransferase -
COG38_RS22990 4503240..4503980 - 741 WP_001225679.1 murein L,D-transpeptidase -
COG38_RS22995 4504136..4504414 - 279 WP_024171431.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
COG38_RS23000 4504417..4504677 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
COG38_RS23005 4504887..4505636 + 750 WP_000056850.1 C40 family peptidase -
COG38_RS23010 4505812..4506309 + 498 WP_001612413.1 REP-associated tyrosine transposase RayT -
COG38_RS23015 4506496..4508208 - 1713 Protein_4208 flagellar biosynthesis protein FlhA -
COG38_RS23020 4508180..4508965 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - tssA / gmhA/lpcA 4485938..4506309 20371


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10829.68 Da        Isoelectric Point: 10.3349

>T87388 WP_024171431.1 NZ_CP024263:c4504414-4504136 [Escherichia coli O169:H41]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVKPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87388 NZ_CP024263:c4504414-4504136 [Escherichia coli O169:H41]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCAAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87388 WP_000729704.1 NZ_CP024263:c4504677-4504417 [Escherichia coli O169:H41]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87388 NZ_CP024263:c4504677-4504417 [Escherichia coli O169:H41]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W4DAB6

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References