Detailed information of TA system TA087354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1902436..1903130
• Replicon: chromosome
• Accession: NZ_CP024260
• Organism: Escherichia coli strain F5656C1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: A4V61_RS10130
• Protein ID: WP_001263493.1
• Coordinates: 1902436..1902834 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A4V61_RS10135
• Protein ID: WP_000554757.1
• Coordinates: 1902837..1903130 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4V61_RS10090	1897707..1898165	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A4V61_RS10095	1898426..1899883	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
A4V61_RS10100	1899940..1900461	-	522	Protein_1827	peptide chain release factor H	-
A4V61_RS10105	1900460..1900663	-	204	Protein_1828	RNA ligase RtcB family protein	-
A4V61_RS10110	1900909..1901184	-	276	Protein_1829	GNAT family N-acetyltransferase	-
A4V61_RS10115	1901245..1902213	-	969	WP_023486738.1	IS5 family transposase	-
A4V61_RS10125	1902241..1902426	-	186	Protein_1831	GNAT family N-acetyltransferase	-
A4V61_RS10130	1902436..1902834	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A4V61_RS10135	1902837..1903130	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A4V61_RS10140	1903182..1904237	-	1056	WP_001226164.1	DNA polymerase IV	-
A4V61_RS10145	1904308..1905231	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
A4V61_RS10150	1905234..1906097	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
A4V61_RS10155	1906110..1906829	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
A4V61_RS10160	1906849..1907316	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1871088..1903130	32042
	-	flank	IS/Tn	-	-	1901245..1902168	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87354 WP_001263493.1 NZ_CP024260:c1902834-1902436 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87354 NZ_CP024260:c1902834-1902436 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87354 WP_000554757.1 NZ_CP024260:c1903130-1902837 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87354 NZ_CP024260:c1903130-1902837 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6