Detailed information of TA system TA087339

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68868..69285
• Replicon: plasmid unnamed2
• Accession: NZ_CP024259
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: COG37_RS26605
• Protein ID: WP_001312861.1
• Coordinates: 69127..69285 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68868..69062 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS26580	65066..65626	-	561	Protein_70	IS3 family transposase	-
COG37_RS26585	65688..67651	+	1964	Protein_71	ParB/RepB/Spo0J family partition protein	-
COG37_RS26590	67706..68140	+	435	WP_000845954.1	conjugation system SOS inhibitor PsiB	-
COG37_RS26595	68137..68856	+	720	WP_001734594.1	plasmid SOS inhibition protein A	-
-	68868..69062	+	195	NuclAT_0	-	Antitoxin
-	68868..69062	+	195	NuclAT_0	-	Antitoxin
-	68868..69062	+	195	NuclAT_0	-	Antitoxin
-	68868..69062	+	195	NuclAT_0	-	Antitoxin
COG37_RS26605	69127..69285	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
COG37_RS26610	69579..71150	-	1572	WP_099554548.1	IS66 family transposase	-
COG37_RS26615	71170..71517	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
COG37_RS26620	71517..72194	-	678	WP_099554551.1	IS66-like element accessory protein TnpA	-
COG37_RS27235	72504..72695	+	192	Protein_78	hypothetical protein	-
COG37_RS26635	72748..73425	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
COG37_RS26640	73425..73772	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltB / eltA / cssA	1..94817	94817
	-	inside	IScluster/Tn	-	cssA	58927..89380	30453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T87339 WP_001312861.1 NZ_CP024259:69127-69285 [Escherichia coli O25:H16]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T87339 NZ_CP024259:69127-69285 [Escherichia coli O25:H16]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT87339 NZ_CP024259:68868-69062 [Escherichia coli O25:H16]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin