Detailed information of TA system TA087337

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40081..40308
• Replicon: plasmid unnamed2
• Accession: NZ_CP024259
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: COG37_RS26435
• Protein ID: WP_032199711.1
• Coordinates: 40081..40260 (-)
• Length: 60 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40252..40308 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS26400	35692..35766	-	75	WP_001365705.1	RepA leader peptide Tap	-
COG37_RS26410	35991..36251	-	261	WP_000083817.1	replication regulatory protein RepA	-
COG37_RS26420	36775..38139	+	1365	Protein_40	IS21-like element ISEc12 family transposase	-
COG37_RS26430	39450..40010	+	561	Protein_42	IS21-like element ISEc12 family helper ATPase IstB	-
COG37_RS26435	40081..40260	-	180	WP_032199711.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40252..40308	+	57	NuclAT_1	-	Antitoxin
-	40252..40308	+	57	NuclAT_1	-	Antitoxin
-	40252..40308	+	57	NuclAT_1	-	Antitoxin
-	40252..40308	+	57	NuclAT_1	-	Antitoxin
COG37_RS26445	40552..40734	+	183	WP_001393357.1	hypothetical protein	-
COG37_RS26450	40958..41689	-	732	WP_001734655.1	type-F conjugative transfer system pilin acetylase TraX	-
COG37_RS26455	41686..42252	-	567	WP_024169751.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltB / eltA / cssA	1..94817	94817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-55)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6745.13 Da
• Isoelectric Point: 8.3561

>T87337 WP_032199711.1 NZ_CP024259:c40260-40081 [Escherichia coli O25:H16]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNCVLP

Nucleotide

• Length: 180 bp

>T87337 NZ_CP024259:c40260-40081 [Escherichia coli O25:H16]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATTGCGTATTACCGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT87337 NZ_CP024259:40252-40308 [Escherichia coli O25:H16]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin