Detailed information of TA system TA087333

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47988..48401
• Replicon: plasmid unnamed1
• Accession: NZ_CP024258
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: COG37_RS25855
• Protein ID: WP_000775238.1
• Coordinates: 47988..48149 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	48201..48401 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS25815	43362..44051	-	690	WP_000283390.1	conjugal transfer transcriptional regulator TraJ	-
COG37_RS25820	44238..44621	-	384	WP_001151524.1	relaxosome protein TraM	-
COG37_RS25825	44943..45545	+	603	WP_077631932.1	transglycosylase SLT domain-containing protein	-
COG37_RS25830	45842..46660	-	819	WP_074481281.1	DUF945 domain-containing protein	-
COG37_RS25835	46769..47065	-	297	WP_001272248.1	hypothetical protein	-
COG37_RS25855	47988..48149	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	48201..48401	-	201	NuclAT_0	-	Antitoxin
-	48201..48401	-	201	NuclAT_0	-	Antitoxin
-	48201..48401	-	201	NuclAT_0	-	Antitoxin
-	48201..48401	-	201	NuclAT_0	-	Antitoxin
COG37_RS25860	48358..48672	-	315	WP_000872086.1	hypothetical protein	-
COG37_RS25865	48669..49388	-	720	WP_001276220.1	plasmid SOS inhibition protein A	-
COG37_RS25870	49385..49819	-	435	WP_000845955.1	conjugation system SOS inhibitor PsiB	-
COG37_RS25875	49874..51838	-	1965	WP_074481283.1	ParB/RepB/Spo0J family partition protein	-
COG37_RS25880	51899..52132	-	234	WP_000005975.1	DUF905 domain-containing protein	-
COG37_RS25885	52189..52716	-	528	WP_074481284.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..96607	96607

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T87333 WP_000775238.1 NZ_CP024258:c48149-47988 [Escherichia coli O25:H16]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T87333 NZ_CP024258:c48149-47988 [Escherichia coli O25:H16]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 201 bp

>AT87333 NZ_CP024258:c48401-48201 [Escherichia coli O25:H16]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGTGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCTA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin