Detailed information of TA system TA087331

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2796..3050
• Replicon: plasmid unnamed1
• Accession: NZ_CP024258
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: COG37_RS25555
• Protein ID: WP_001362723.1
• Coordinates: 2796..3002 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	2989..3050 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS27200	218..397	-	180	WP_074483066.1	hypothetical protein	-
COG37_RS25535	1097..1954	-	858	WP_074480778.1	incFII family plasmid replication initiator RepA	-
COG37_RS25540	1947..2021	-	75	WP_077135826.1	RepA leader peptide Tap	-
COG37_RS25550	2255..2512	-	258	WP_000083826.1	replication regulatory protein RepA	-
COG37_RS25555	2796..3002	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2989..3050	+	62	NuclAT_1	-	Antitoxin
-	2989..3050	+	62	NuclAT_1	-	Antitoxin
-	2989..3050	+	62	NuclAT_1	-	Antitoxin
-	2989..3050	+	62	NuclAT_1	-	Antitoxin
COG37_RS25565	3202..3666	-	465	WP_001756974.1	hypothetical protein	-
COG37_RS25570	3821..4411	-	591	WP_074480776.1	DUF2726 domain-containing protein	-
COG37_RS25575	4449..4658	-	210	WP_001353639.1	transcriptional regulator	-
COG37_RS25580	4704..5105	-	402	WP_077760680.1	thermonuclease family protein	-
COG37_RS25590	5162..5839	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
COG37_RS25595	5839..6186	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
COG37_RS25600	6206..7777	+	1572	WP_000381395.1	IS66 family transposase	-
COG37_RS25605	7804..7935	-	132	WP_099558417.1	nuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..96607	96607

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T87331 WP_001362723.1 NZ_CP024258:c3002-2796 [Escherichia coli O25:H16]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87331 NZ_CP024258:c3002-2796 [Escherichia coli O25:H16]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATAGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT87331 NZ_CP024258:2989-3050 [Escherichia coli O25:H16]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin