Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4116730..4117271 Replicon chromosome
Accession NZ_CP024257
Organism Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag COG37_RS21605 Protein ID WP_000615976.1
Coordinates 4116993..4117271 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag COG37_RS21600 Protein ID WP_000729704.1
Coordinates 4116730..4116990 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG37_RS21580 4112405..4113190 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
COG37_RS21585 4113162..4114874 + 1713 Protein_3929 flagellar biosynthesis protein FlhA -
COG37_RS21590 4115098..4115595 - 498 WP_000006242.1 REP-associated tyrosine transposase RayT -
COG37_RS21595 4115771..4116544 - 774 WP_016245244.1 C40 family peptidase -
COG37_RS21600 4116730..4116990 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
COG37_RS21605 4116993..4117271 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
COG37_RS21610 4117427..4118167 + 741 WP_001727406.1 murein L,D-transpeptidase -
COG37_RS21615 4118138..4118905 - 768 WP_001365365.1 class II glutamine amidotransferase -
COG37_RS21620 4119111..4119689 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T87329 WP_000615976.1 NZ_CP024257:4116993-4117271 [Escherichia coli O25:H16]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87329 NZ_CP024257:4116993-4117271 [Escherichia coli O25:H16]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87329 WP_000729704.1 NZ_CP024257:4116730-4116990 [Escherichia coli O25:H16]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87329 NZ_CP024257:4116730-4116990 [Escherichia coli O25:H16]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References