Detailed information of TA system TA087328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4110533..4111227
• Replicon: chromosome
• Accession: NZ_CP024257
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: COG37_RS21565
• Protein ID: WP_001263493.1
• Coordinates: 4110533..4110931 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: COG37_RS21570
• Protein ID: WP_000554757.1
• Coordinates: 4110934..4111227 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS21535	4105534..4106778	-	1245	WP_000189541.1	esterase FrsA	-
-	4106194..4106274	-	81	NuclAT_12	-	-
-	4106194..4106274	-	81	NuclAT_12	-	-
-	4106194..4106274	-	81	NuclAT_12	-	-
-	4106194..4106274	-	81	NuclAT_12	-	-
COG37_RS21540	4106870..4107328	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
COG37_RS21545	4107589..4109045	+	1457	Protein_3921	cytosol nonspecific dipeptidase	-
COG37_RS21550	4109102..4109623	-	522	Protein_3922	peptide chain release factor H	-
COG37_RS21555	4109622..4109825	-	204	Protein_3923	RNA ligase RtcB family protein	-
COG37_RS21560	4110071..4110523	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
COG37_RS21565	4110533..4110931	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
COG37_RS21570	4110934..4111227	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
COG37_RS21575	4111279..4112334	-	1056	WP_001226164.1	DNA polymerase IV	-
COG37_RS21580	4112405..4113190	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
COG37_RS21585	4113162..4114874	+	1713	Protein_3929	flagellar biosynthesis protein FlhA	-
COG37_RS21590	4115098..4115595	-	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87328 WP_001263493.1 NZ_CP024257:c4110931-4110533 [Escherichia coli O25:H16]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87328 NZ_CP024257:c4110931-4110533 [Escherichia coli O25:H16]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87328 WP_000554757.1 NZ_CP024257:c4111227-4110934 [Escherichia coli O25:H16]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87328 NZ_CP024257:c4111227-4110934 [Escherichia coli O25:H16]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6