Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2163437..2163939 Replicon chromosome
Accession NZ_CP024257
Organism Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag COG37_RS11030 Protein ID WP_000767829.1
Coordinates 2163685..2163939 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag COG37_RS11025 Protein ID WP_001259255.1
Coordinates 2163437..2163688 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG37_RS10995 2158615..2159682 - 1068 WP_000080145.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
COG37_RS11000 2159682..2160752 - 1071 WP_000108954.1 histidinol-phosphate transaminase -
COG37_RS11005 2160755..2162053 - 1299 WP_001735396.1 histidinol dehydrogenase -
COG37_RS11010 2162059..2162958 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
COG37_RS11020 2163104..2163154 - 51 WP_001364200.1 his operon leader peptide -
COG37_RS11025 2163437..2163688 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
COG37_RS11030 2163685..2163939 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
COG37_RS11035 2164022..2164846 + 825 WP_000754737.1 SDR family oxidoreductase -
COG37_RS11040 2164892..2165821 + 930 WP_000803337.1 LysR family transcriptional regulator -
COG37_RS11045 2166036..2166098 + 63 WP_010723108.1 membrane protein YoeI -
COG37_RS11050 2166088..2167446 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
COG37_RS11055 2167625..2168683 + 1059 WP_000492340.1 transport protein YeeE -
COG37_RS11060 2168697..2168924 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87316 WP_000767829.1 NZ_CP024257:2163685-2163939 [Escherichia coli O25:H16]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87316 NZ_CP024257:2163685-2163939 [Escherichia coli O25:H16]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87316 WP_001259255.1 NZ_CP024257:2163437-2163688 [Escherichia coli O25:H16]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87316 NZ_CP024257:2163437-2163688 [Escherichia coli O25:H16]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References