Detailed information of TA system TA087311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 887351..888150
• Replicon: chromosome
• Accession: NZ_CP024257
• Organism: Escherichia coli O25:H16 strain F5505-C1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: COG37_RS04565
• Protein ID: WP_000347273.1
• Coordinates: 887351..887815 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: COG37_RS04570
• Protein ID: WP_005762317.1
• Coordinates: 887815..888150 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG37_RS04530	882352..882786	-	435	WP_000948842.1	PTS sugar transporter subunit IIA	-
COG37_RS04535	882804..883682	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
COG37_RS04540	883672..884451	-	780	WP_000406208.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
COG37_RS04545	884462..884935	-	474	WP_001735764.1	PTS N-acetylgalactosamine transporter subunit IIB	-
COG37_RS04550	884958..886238	-	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
COG37_RS04560	886487..887296	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
COG37_RS04565	887351..887815	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
COG37_RS04570	887815..888150	-	336	WP_005762317.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
COG37_RS04575	888299..889870	-	1572	WP_001273753.1	galactarate dehydratase	-
COG37_RS04580	890245..891564	+	1320	WP_001735763.1	galactarate/glucarate/glycerate transporter GarP	-
COG37_RS04585	891580..892350	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T87311 WP_000347273.1 NZ_CP024257:c887815-887351 [Escherichia coli O25:H16]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T87311 NZ_CP024257:c887815-887351 [Escherichia coli O25:H16]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.91 Da
• Isoelectric Point: 4.8616

>AT87311 WP_005762317.1 NZ_CP024257:c888150-887815 [Escherichia coli O25:H16]
MPANARSHAVLTSESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT87311 NZ_CP024257:c888150-887815 [Escherichia coli O25:H16]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCTCTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7