Detailed information of TA system TA087296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1876393..1877087
• Replicon: chromosome
• Accession: NZ_CP024256
• Organism: Escherichia coli strain ATCC 43886

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CS032_RS11105
• Protein ID: WP_001263493.1
• Coordinates: 1876689..1877087 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CS032_RS11100
• Protein ID: WP_000554757.1
• Coordinates: 1876393..1876686 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS032_RS11080	1872026..1872523	+	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-
CS032_RS11085	1872747..1874459	-	1713	Protein_1738	flagellar biosynthesis protein FlhA	-
CS032_RS11090	1874431..1875216	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
CS032_RS11095	1875287..1876341	+	1055	Protein_1740	DNA polymerase IV	-
CS032_RS11100	1876393..1876686	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CS032_RS11105	1876689..1877087	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CS032_RS11110	1877097..1877549	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CS032_RS11115	1877795..1877998	+	204	Protein_1744	RNA ligase RtcB family protein	-
CS032_RS11120	1877997..1878518	+	522	Protein_1745	peptide chain release factor H	-
CS032_RS11125	1878575..1880032	-	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
CS032_RS11130	1880293..1880751	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CS032_RS11135	1880843..1882087	+	1245	WP_032200258.1	esterase FrsA	-
-	1881347..1881427	+	81	NuclAT_11	-	-
-	1881347..1881427	+	81	NuclAT_11	-	-
-	1881347..1881427	+	81	NuclAT_11	-	-
-	1881347..1881427	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87296 WP_001263493.1 NZ_CP024256:1876689-1877087 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87296 NZ_CP024256:1876689-1877087 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87296 WP_000554757.1 NZ_CP024256:1876393-1876686 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87296 NZ_CP024256:1876393-1876686 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6