Detailed information of TA system TA087287

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77476..77893
• Replicon: plasmid unnamed2
• Accession: NZ_CP024255
• Organism: Escherichia coli strain ATCC 43886

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CS032_RS01315
• Protein ID: WP_001312861.1
• Coordinates: 77735..77893 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77476..77670 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS032_RS01290	73674..74234	-	561	Protein_79	IS3 family transposase	-
CS032_RS01295	74296..76259	+	1964	Protein_80	ParB/RepB/Spo0J family partition protein	-
CS032_RS01300	76314..76748	+	435	WP_000845954.1	conjugation system SOS inhibitor PsiB	-
CS032_RS01305	76745..77464	+	720	WP_001734594.1	plasmid SOS inhibition protein A	-
-	77476..77670	+	195	NuclAT_0	-	Antitoxin
-	77476..77670	+	195	NuclAT_0	-	Antitoxin
-	77476..77670	+	195	NuclAT_0	-	Antitoxin
-	77476..77670	+	195	NuclAT_0	-	Antitoxin
CS032_RS01315	77735..77893	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CS032_RS01320	78187..79758	-	1572	WP_099554548.1	IS66 family transposase	-
CS032_RS01325	79778..80125	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CS032_RS01330	80125..80802	-	678	WP_099554551.1	IS66-like element accessory protein TnpA	-
CS032_RS27905	81112..81303	+	192	Protein_87	hypothetical protein	-
CS032_RS01345	81356..82033	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CS032_RS01350	82033..82380	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltB / eltA / cssA	1..95515	95515
	-	inside	IScluster/Tn	-	-	73674..92036	18362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T87287 WP_001312861.1 NZ_CP024255:77735-77893 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T87287 NZ_CP024255:77735-77893 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT87287 NZ_CP024255:77476-77670 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin