Detailed information of TA system TA087279

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33190..33602
• Replicon: plasmid unnamed3
• Accession: NZ_CP024248
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: CQS47_RS25245
• Protein ID: WP_000775238.1
• Coordinates: 33190..33351 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	33417..33602 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS25200	28524..28739	-	216	WP_024191430.1	hypothetical protein	-
CQS47_RS25210	29081..29323	-	243	WP_000540593.1	hypothetical protein	-
CQS47_RS25215	29283..29645	-	363	WP_000107524.1	hypothetical protein	-
CQS47_RS25225	30130..31665	+	1536	WP_099590317.1	IS21 family transposase	-
CQS47_RS25230	31682..32437	+	756	WP_001282653.1	IS21-like element ISSso4 family helper ATPase IstB	-
CQS47_RS25245	33190..33351	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33417..33602	-	186	NuclAT_0	-	Antitoxin
-	33417..33602	-	186	NuclAT_0	-	Antitoxin
-	33417..33602	-	186	NuclAT_0	-	Antitoxin
-	33417..33602	-	186	NuclAT_0	-	Antitoxin
CQS47_RS25250	33559..33873	-	315	WP_000872086.1	hypothetical protein	-
CQS47_RS25255	33870..34544	-	675	WP_001719371.1	plasmid SOS inhibition protein A	-
CQS47_RS25260	34541..34975	-	435	WP_001719370.1	conjugation system SOS inhibitor PsiB	-
CQS47_RS25265	35030..36997	-	1968	WP_099590318.1	ParB/RepB/Spo0J family partition protein	-
CQS47_RS25270	37058..37291	-	234	WP_001297827.1	DUF905 domain-containing protein	-
CQS47_RS25275	37348..37869	-	522	WP_032201449.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / faeE / faeD / cofA / cssA	1..138289	138289

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T87279 WP_000775238.1 NZ_CP024248:c33351-33190 [Escherichia coli O27:H7]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T87279 NZ_CP024248:c33351-33190 [Escherichia coli O27:H7]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 186 bp

>AT87279 NZ_CP024248:c33602-33417 [Escherichia coli O27:H7]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGTGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAAGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin