Detailed information of TA system TA087277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3796..4050
• Replicon: plasmid unnamed3
• Accession: NZ_CP024248
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CQS47_RS25000
• Protein ID: WP_001362723.1
• Coordinates: 3796..4002 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	3994..4050 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS24960	191..837	-	647	Protein_0	IS630 family transposase	-
CQS47_RS24965	838..1239	+	402	Protein_1	IS91 family transposase	-
CQS47_RS24980	2102..2959	-	858	WP_001401515.1	incFII family plasmid replication initiator RepA	-
CQS47_RS24985	2952..3026	-	75	WP_001365705.1	RepA leader peptide Tap	-
CQS47_RS24995	3252..3512	-	261	WP_000083817.1	replication regulatory protein RepA	-
CQS47_RS25000	3796..4002	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3994..4050	+	57	NuclAT_1	-	Antitoxin
-	3994..4050	+	57	NuclAT_1	-	Antitoxin
-	3994..4050	+	57	NuclAT_1	-	Antitoxin
-	3994..4050	+	57	NuclAT_1	-	Antitoxin
CQS47_RS25010	4294..4476	+	183	WP_001393357.1	hypothetical protein	-
CQS47_RS25015	4700..5431	-	732	WP_001595201.1	type-F conjugative transfer system pilin acetylase TraX	-
CQS47_RS25020	5428..5993	-	566	Protein_8	DUF2726 domain-containing protein	-
CQS47_RS25025	6011..7207	-	1197	WP_024191432.1	conjugal transfer protein TraI	-
CQS47_RS25030	7303..7551	+	249	Protein_10	transposase	-
CQS47_RS25035	7616..8767	+	1152	WP_001595195.1	IS30-like element IS30 family transposase	-
CQS47_RS26455	8848..9003	+	156	WP_000074142.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / faeE / faeD / cofA / cssA	1..138289	138289
	-	flank	IS/Tn	-	-	1006..1242	236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T87277 WP_001362723.1 NZ_CP024248:c4002-3796 [Escherichia coli O27:H7]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87277 NZ_CP024248:c4002-3796 [Escherichia coli O27:H7]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACCTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT87277 NZ_CP024248:3994-4050 [Escherichia coli O27:H7]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCCTTTCT

Structures

Antitoxin