Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4493818..4494359 Replicon chromosome
Accession NZ_CP024245
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CQS47_RS23255 Protein ID WP_000615976.1
Coordinates 4494081..4494359 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CQS47_RS23250 Protein ID WP_000729704.1
Coordinates 4493818..4494078 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS23225 4489601..4490386 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
CQS47_RS23230 4490358..4492070 + 1713 Protein_4225 flagellar biosynthesis protein FlhA -
CQS47_RS23235 4492175..4492453 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CQS47_RS23240 4492446..4492802 + 357 WP_001030486.1 type II toxin-antitoxin system HicB family antitoxin -
CQS47_RS23245 4492859..4493632 - 774 WP_000543897.1 C40 family peptidase -
CQS47_RS23250 4493818..4494078 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CQS47_RS23255 4494081..4494359 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CQS47_RS23260 4494515..4495255 + 741 WP_001225679.1 murein L,D-transpeptidase -
CQS47_RS23265 4495226..4495993 - 768 WP_000333380.1 class II glutamine amidotransferase -
CQS47_RS23270 4496199..4496777 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T87275 WP_000615976.1 NZ_CP024245:4494081-4494359 [Escherichia coli O27:H7]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87275 NZ_CP024245:4494081-4494359 [Escherichia coli O27:H7]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87275 WP_000729704.1 NZ_CP024245:4493818-4494078 [Escherichia coli O27:H7]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87275 NZ_CP024245:4493818-4494078 [Escherichia coli O27:H7]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References