Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2636043..2636545 Replicon chromosome
Accession NZ_CP024245
Organism Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CQS47_RS13345 Protein ID WP_000767829.1
Coordinates 2636291..2636545 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CQS47_RS13340 Protein ID WP_001259255.1
Coordinates 2636043..2636294 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CQS47_RS13310 2631220..2632287 - 1068 WP_000080108.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CQS47_RS13315 2632287..2633357 - 1071 WP_000108955.1 histidinol-phosphate transaminase -
CQS47_RS13320 2633354..2634658 - 1305 WP_029488616.1 histidinol dehydrogenase -
CQS47_RS13325 2634664..2635563 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CQS47_RS13335 2635709..2635759 - 51 WP_001364200.1 his operon leader peptide -
CQS47_RS13340 2636043..2636294 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CQS47_RS13345 2636291..2636545 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CQS47_RS13350 2636628..2637452 + 825 WP_021562277.1 SDR family oxidoreductase -
CQS47_RS13355 2637498..2638427 + 930 WP_000803351.1 LysR family transcriptional regulator -
CQS47_RS13360 2638642..2638704 + 63 WP_010723108.1 membrane protein YoeI -
CQS47_RS13365 2638694..2640052 + 1359 WP_096842198.1 putrescine/proton symporter PlaP -
CQS47_RS13370 2640231..2641289 + 1059 WP_000492340.1 transport protein YeeE -
CQS47_RS13375 2641303..2641530 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87262 WP_000767829.1 NZ_CP024245:2636291-2636545 [Escherichia coli O27:H7]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87262 NZ_CP024245:2636291-2636545 [Escherichia coli O27:H7]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87262 WP_001259255.1 NZ_CP024245:2636043-2636294 [Escherichia coli O27:H7]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87262 NZ_CP024245:2636043-2636294 [Escherichia coli O27:H7]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References