Detailed information of TA system TA087257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 605489..606091
• Replicon: chromosome
• Accession: NZ_CP024245
• Organism: Escherichia coli O27:H7 strain B4103-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CQS47_RS03135
• Protein ID: WP_000897305.1
• Coordinates: 605780..606091 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CQS47_RS03130
• Protein ID: WP_000356397.1
• Coordinates: 605489..605779 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQS47_RS03095	601037..601672	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CQS47_RS03100	601669..602598	+	930	WP_001594636.1	formate dehydrogenase accessory protein FdhE	-
CQS47_RS03110	602997..603977	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
CQS47_RS03115	604206..604448	-	243	WP_001086388.1	hypothetical protein	-
CQS47_RS03120	604666..604884	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CQS47_RS03130	605489..605779	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CQS47_RS03135	605780..606091	-	312	WP_000897305.1	hypothetical protein	Toxin
CQS47_RS03140	606320..607228	+	909	WP_001162704.1	alpha/beta hydrolase	-
CQS47_RS03145	607292..608233	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
CQS47_RS03150	608278..608715	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CQS47_RS03155	608712..609584	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CQS47_RS03160	609578..610177	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	602997..603977	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87257 WP_000897305.1 NZ_CP024245:c606091-605780 [Escherichia coli O27:H7]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87257 NZ_CP024245:c606091-605780 [Escherichia coli O27:H7]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87257 WP_000356397.1 NZ_CP024245:c605779-605489 [Escherichia coli O27:H7]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87257 NZ_CP024245:c605779-605489 [Escherichia coli O27:H7]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7