Detailed information of TA system TA087232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1960188..1960790
• Replicon: chromosome
• Accession: NZ_CP024243
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: COG34_RS10190
• Protein ID: WP_000897305.1
• Coordinates: 1960479..1960790 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: COG34_RS10185
• Protein ID: WP_000356397.1
• Coordinates: 1960188..1960478 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS10155	1956113..1957015	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
COG34_RS10160	1957012..1957647	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
COG34_RS10165	1957644..1958573	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
COG34_RS10170	1958903..1959145	-	243	WP_001087409.1	hypothetical protein	-
COG34_RS10175	1959365..1959583	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
COG34_RS10185	1960188..1960478	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
COG34_RS10190	1960479..1960790	-	312	WP_000897305.1	hypothetical protein	Toxin
COG34_RS10195	1961019..1961927	+	909	WP_001162704.1	alpha/beta hydrolase	-
COG34_RS10200	1961991..1962932	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
COG34_RS10205	1962977..1963414	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
COG34_RS10210	1963411..1964283	-	873	WP_000920762.1	virulence factor BrkB family protein	-
COG34_RS10215	1964277..1964876	-	600	WP_001295269.1	glucose-1-phosphatase	-
COG34_RS10220	1964975..1965760	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87232 WP_000897305.1 NZ_CP024243:c1960790-1960479 [Escherichia coli O128:H27]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87232 NZ_CP024243:c1960790-1960479 [Escherichia coli O128:H27]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87232 WP_000356397.1 NZ_CP024243:c1960478-1960188 [Escherichia coli O128:H27]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87232 NZ_CP024243:c1960478-1960188 [Escherichia coli O128:H27]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7