Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1067458..1067999 Replicon chromosome
Accession NZ_CP024243
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag COG34_RS05645 Protein ID WP_000615976.1
Coordinates 1067721..1067999 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag COG34_RS05640 Protein ID WP_000729704.1
Coordinates 1067458..1067718 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS05615 1063241..1064026 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
COG34_RS05620 1063998..1065710 + 1713 Protein_1014 flagellar biosynthesis protein FlhA -
COG34_RS05625 1065815..1066093 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
COG34_RS05630 1066086..1066442 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
COG34_RS05635 1066499..1067272 - 774 WP_000543895.1 C40 family peptidase -
COG34_RS05640 1067458..1067718 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
COG34_RS05645 1067721..1067999 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
COG34_RS05650 1068155..1068895 + 741 WP_001225679.1 murein L,D-transpeptidase -
COG34_RS05655 1068866..1069633 - 768 WP_000333380.1 class II glutamine amidotransferase -
COG34_RS05660 1069839..1070417 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T87227 WP_000615976.1 NZ_CP024243:1067721-1067999 [Escherichia coli O128:H27]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87227 NZ_CP024243:1067721-1067999 [Escherichia coli O128:H27]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87227 WP_000729704.1 NZ_CP024243:1067458-1067718 [Escherichia coli O128:H27]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87227 NZ_CP024243:1067458-1067718 [Escherichia coli O128:H27]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References