Detailed information of TA system TA087221

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 94062..94326
• Replicon: plasmid unnamed1
• Accession: NZ_CP024241
• Organism: Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: COG33_RS26445
• Protein ID: WP_001704300.1
• Coordinates: 94062..94214 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	94264..94326 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG33_RS26420	89544..91712	+	2169	WP_001388820.1	IncI1-type conjugal transfer membrane protein TraY	-
COG33_RS27355	91788..92386	+	599	Protein_101	plasmid IncI1-type surface exclusion protein ExcA	-
COG33_RS26430	92484..92693	+	210	WP_000416064.1	hemolysin expression modulator Hha	-
COG33_RS27605	92902..93078	+	177	WP_001054897.1	hypothetical protein	-
COG33_RS26435	93143..93439	-	297	WP_001275298.1	hypothetical protein	-
COG33_RS26440	93739..93990	+	252	WP_001291963.1	hypothetical protein	-
COG33_RS26445	94062..94214	-	153	WP_001704300.1	Hok/Gef family protein	Toxin
-	94264..94326	+	63	NuclAT_0	-	Antitoxin
-	94264..94326	+	63	NuclAT_0	-	Antitoxin
-	94264..94326	+	63	NuclAT_0	-	Antitoxin
-	94264..94326	+	63	NuclAT_0	-	Antitoxin
-	94567..94622	+	56	NuclAT_1	-	-
-	94567..94622	+	56	NuclAT_1	-	-
-	94567..94622	+	56	NuclAT_1	-	-
-	94567..94622	+	56	NuclAT_1	-	-
COG33_RS26450	94783..95769	+	987	WP_000987364.1	hypothetical protein	-
COG33_RS26455	95986..97194	+	1209	WP_001388819.1	IncI1-type conjugal transfer protein TrbA	-
COG33_RS26460	97213..98283	+	1071	WP_000151586.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltA / eltB / csvA	1..104674	104674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5809.14 Da
• Isoelectric Point: 8.7948

>T87221 WP_001704300.1 NZ_CP024241:c94214-94062 [Escherichia coli O114:H49]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVFVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87221 NZ_CP024241:c94214-94062 [Escherichia coli O114:H49]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGTTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT87221 NZ_CP024241:94264-94326 [Escherichia coli O114:H49]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin