Detailed information of TA system TA087214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3275988..3276590
• Replicon: chromosome
• Accession: NZ_CP024240
• Organism: Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: COG33_RS17345
• Protein ID: WP_000897305.1
• Coordinates: 3275988..3276299 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: COG33_RS17350
• Protein ID: WP_000356397.1
• Coordinates: 3276300..3276590 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG33_RS17315	3271018..3271803	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
COG33_RS17320	3271902..3272501	+	600	WP_001315111.1	glucose-1-phosphatase	-
COG33_RS17325	3272495..3273367	+	873	WP_000920762.1	virulence factor BrkB family protein	-
COG33_RS17330	3273364..3273801	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
COG33_RS17335	3273846..3274787	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
COG33_RS17340	3274851..3275759	-	909	WP_001162704.1	alpha/beta hydrolase	-
COG33_RS17345	3275988..3276299	+	312	WP_000897305.1	hypothetical protein	Toxin
COG33_RS17350	3276300..3276590	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
COG33_RS17360	3277195..3277413	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
COG33_RS17365	3277632..3277874	+	243	WP_001086388.1	hypothetical protein	-
COG33_RS17370	3278204..3279133	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
COG33_RS17375	3279130..3279765	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
COG33_RS17380	3279762..3280664	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87214 WP_000897305.1 NZ_CP024240:3275988-3276299 [Escherichia coli O114:H49]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87214 NZ_CP024240:3275988-3276299 [Escherichia coli O114:H49]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87214 WP_000356397.1 NZ_CP024240:3276300-3276590 [Escherichia coli O114:H49]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87214 NZ_CP024240:3276300-3276590 [Escherichia coli O114:H49]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7