Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3938964..3939658 | Replicon | chromosome |
| Accession | NZ_CP024239 | ||
| Organism | Escherichia coli O15:H11 strain 90-9272 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | COG31_RS22300 | Protein ID | WP_001263493.1 |
| Coordinates | 3939260..3939658 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | COG31_RS22295 | Protein ID | WP_000554757.1 |
| Coordinates | 3938964..3939257 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| COG31_RS28540 (3934295) | 3934295..3934576 | - | 282 | WP_249929713.1 | hypothetical protein | - |
| COG31_RS22275 (3934874) | 3934874..3935896 | + | 1023 | WP_000255944.1 | IS21-like element IS100 family transposase | - |
| COG31_RS22280 (3935893) | 3935893..3936675 | + | 783 | WP_001300609.1 | IS21-like element IS100kyp family helper ATPase IstB | - |
| COG31_RS22285 (3936719) | 3936719..3937798 | - | 1080 | WP_099550845.1 | recombinase family protein | - |
| COG31_RS22290 (3937860) | 3937860..3938912 | + | 1053 | WP_000691315.1 | DNA polymerase IV | - |
| COG31_RS22295 (3938964) | 3938964..3939257 | + | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| COG31_RS22300 (3939260) | 3939260..3939658 | + | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| COG31_RS22305 (3939668) | 3939668..3940120 | + | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| COG31_RS22310 (3940366) | 3940366..3940569 | + | 204 | Protein_3766 | RtcB family protein | - |
| COG31_RS22315 (3940568) | 3940568..3941089 | + | 522 | Protein_3767 | peptide chain release factor H | - |
| COG31_RS22320 (3941146) | 3941146..3942601 | - | 1456 | Protein_3768 | cytosol nonspecific dipeptidase | - |
| COG31_RS22325 (3942862) | 3942862..3943320 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| - (3943916) | 3943916..3943996 | + | 81 | NuclAT_11 | - | - |
| - (3943916) | 3943916..3943996 | + | 81 | NuclAT_11 | - | - |
| - (3943916) | 3943916..3943996 | + | 81 | NuclAT_11 | - | - |
| - (3943916) | 3943916..3943996 | + | 81 | NuclAT_11 | - | - |
| COG31_RS22330 (3943412) | 3943412..3944655 | + | 1244 | Protein_3770 | esterase FrsA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T87195 WP_001263493.1 NZ_CP024239:3939260-3939658 [Escherichia coli O15:H11]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T87195 NZ_CP024239:3939260-3939658 [Escherichia coli O15:H11]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT87195 WP_000554757.1 NZ_CP024239:3938964-3939257 [Escherichia coli O15:H11]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT87195 NZ_CP024239:3938964-3939257 [Escherichia coli O15:H11]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|