Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3547846..3548573 Replicon chromosome
Accession NZ_CP024239
Organism Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag COG31_RS20280 Protein ID WP_000550189.1
Coordinates 3547846..3548160 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag COG31_RS20285 Protein ID WP_000560266.1
Coordinates 3548157..3548573 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG31_RS20255 (3544004) 3544004..3544990 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
COG31_RS20260 (3545069) 3545069..3545761 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
COG31_RS20265 (3545838) 3545838..3546341 - 504 WP_001333820.1 M48 family metallopeptidase -
COG31_RS20270 (3546426) 3546426..3547562 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
COG31_RS20280 (3547846) 3547846..3548160 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
COG31_RS20285 (3548157) 3548157..3548573 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
COG31_RS20290 (3548618) 3548618..3550636 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
COG31_RS20295 (3551062) 3551062..3553413 - 2352 WP_099550820.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T87192 WP_000550189.1 NZ_CP024239:3547846-3548160 [Escherichia coli O15:H11]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T87192 NZ_CP024239:3547846-3548160 [Escherichia coli O15:H11]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT87192 WP_000560266.1 NZ_CP024239:3548157-3548573 [Escherichia coli O15:H11]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT87192 NZ_CP024239:3548157-3548573 [Escherichia coli O15:H11]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References