Detailed information of TA system TA087172

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119608..120020
• Replicon: plasmid unnamed
• Accession: NZ_CP024238
• Organism: Escherichia coli O15:H11 strain 90-9272

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: COG31_RS00825
• Protein ID: WP_223163518.1
• Coordinates: 119608..119730 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	119815..120020 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG31_RS00765 (114907)	114907..115554	+	648	WP_023486209.1	transglycosylase SLT domain-containing protein	-
COG31_RS00770 (115850)	115850..116671	-	822	WP_001234441.1	DUF932 domain-containing protein	-
COG31_RS00775 (116782)	116782..117078	-	297	WP_001272251.1	hypothetical protein	-
COG31_RS28075 (117077)	117077..117421	+	345	WP_249929700.1	hypothetical protein	-
COG31_RS28080 (117546)	117546..117806	-	261	WP_071600428.1	hypothetical protein	-
COG31_RS00800 (118072)	118072..118314	-	243	WP_000540588.1	hypothetical protein	-
COG31_RS00805 (118373)	118373..118696	-	324	WP_000533253.1	hypothetical protein	-
COG31_RS28085 (118992)	118992..119370	+	379	Protein_143	hypothetical protein	-
COG31_RS00825 (119608)	119608..119730	-	123	WP_223163518.1	Hok/Gef family protein	Toxin
COG31_RS28090 (119675)	119675..119827	-	153	Protein_145	DUF5431 family protein	-
- (119815)	119815..120020	-	206	NuclAT_0	-	Antitoxin
- (119815)	119815..120020	-	206	NuclAT_0	-	Antitoxin
- (119815)	119815..120020	-	206	NuclAT_0	-	Antitoxin
- (119815)	119815..120020	-	206	NuclAT_0	-	Antitoxin
COG31_RS00830 (119977)	119977..120291	-	315	WP_000872086.1	hypothetical protein	-
COG31_RS00835 (120288)	120288..121007	-	720	WP_001276221.1	plasmid SOS inhibition protein A	-
COG31_RS00840 (121004)	121004..121438	-	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
COG31_RS00845 (121493)	121493..123457	-	1965	WP_001759171.1	ParB/RepB/Spo0J family partition protein	-
COG31_RS00850 (123521)	123521..123754	-	234	WP_000005971.1	DUF905 domain-containing protein	-
COG31_RS00855 (123811)	123811..124338	-	528	WP_000290790.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpA / etpB / espC / eltB / eltA / eltA / eltB / csvA	1..274465	274465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4506.32 Da
• Isoelectric Point: 8.2691

>T87172 WP_223163518.1 NZ_CP024238:c119730-119608 [Escherichia coli O15:H11]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK

Nucleotide

• Length: 123 bp

>T87172 NZ_CP024238:c119730-119608 [Escherichia coli O15:H11]
ATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCGGAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAG
GGAGGTTACGGCAAGTTTGGCTTACGAATCCGGCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 206 bp

>AT87172 NZ_CP024238:c120020-119815 [Escherichia coli O15:H11]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAATGTGGTCAGCGTGCTGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin