Detailed information of TA system TA087166

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11985..12249
• Replicon: plasmid unnamed2
• Accession: NZ_CP024234
• Organism: Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CNZ63_RS25680
• Protein ID: WP_001387489.1
• Coordinates: 12097..12249 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	11985..12047 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	7852..7912	-	61	NuclAT_1	-	-
-	7852..7912	-	61	NuclAT_1	-	-
-	7852..7912	-	61	NuclAT_1	-	-
-	7852..7912	-	61	NuclAT_1	-	-
CNZ63_RS25660	7979..9070	-	1092	WP_000426061.1	hypothetical protein	-
CNZ63_RS25665	9309..9959	+	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
CNZ63_RS25670	9959..10306	+	348	WP_099548426.1	IS66 family insertion sequence element accessory protein TnpB	-
CNZ63_RS25675	10326..11897	+	1572	WP_099548428.1	IS66 family transposase	-
-	11985..12047	-	63	NuclAT_0	-	Antitoxin
-	11985..12047	-	63	NuclAT_0	-	Antitoxin
-	11985..12047	-	63	NuclAT_0	-	Antitoxin
-	11985..12047	-	63	NuclAT_0	-	Antitoxin
CNZ63_RS25680	12097..12249	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CNZ63_RS25685	12321..12572	-	252	WP_001291965.1	hypothetical protein	-
CNZ63_RS25690	12872..13168	+	297	WP_001275298.1	hypothetical protein	-
CNZ63_RS29095	13233..13409	-	177	WP_001054897.1	hypothetical protein	-
CNZ63_RS25695	13801..14010	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CNZ63_RS25700	14082..14732	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
CNZ63_RS25705	14806..16974	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40223	40223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T87166 WP_001387489.1 NZ_CP024234:12097-12249 [Escherichia coli O6:H16]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87166 NZ_CP024234:12097-12249 [Escherichia coli O6:H16]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT87166 NZ_CP024234:c12047-11985 [Escherichia coli O6:H16]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin